![]() Scaling and Display Preferences FlowJo X inherently works with CyTOF data. FlowJo supports cloud deployment for excellent scaling of performance in a demanding, high-throughput environment, and can be integrated into Laboratory Information Management Systems (LIMS). FlowJo has sophisticated tools that allow for generation of statistics, graphs, tables, web pages, mathematical models, and movies. Its strength is in analyzing whole experiments encompassing many related samples. FlowJo ( is the industry leading flow cytometric data analysis software. Each element can be resolved eliminating the need for compensation, and any element absent from a sample can theoretically be used as a reagent. What makes this process so useful is that there is very little noise associated with the ion tags. The measured time of flight is the time it takes particles to fly to the detector. This is done within a magnetic field so that the particles move across the magnetic field to a detector at a rate proportional to the size of the particle the lighter the particle, the faster it moves. Cells labeled with these antibody-isotope conjugates are then vaporized, ionizing the elements. In a mass cytometry experiment instead of tagging antibodies with fluorescent molecules, the tags are isotopes of any element not found naturally in the biological system under study. Background The CyTOF is a mass cytometer produced by DVS Sciences ( The name is an abbreviation for cytometry Time Of Flight, and the machine is used to conduct cytometry experiments on a much larger scale than can be achieved with a traditional fluorescence based cytometer. Heat Mapping and Other Conditional Displays 5. Tools in FlowJo for High-Dimensional Analysis 4.1 Drag and drop clustering 4.2 Multi-graph overlay 4.3 SPADE, Flow Means, and Other Data Mining Tools 4. Scale and transform modification 3.1 Arcsinh Transform 3.2 T-button 4. ![]() Scaling and Display Preferences 2.1 Cytometer Based Preferences 2.2 Useful Display Preferences 3. 1 Using CyTOF Data with FlowJo Version Revised 2/3/14ΔΆ Table of Contents 1.
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